Part:BBa_K1100100:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1100100

The stochastic dynamics of the constitutive promoter family by 2013 Fudan iGEM team

We fused the constitutive promoters to a sfGFP reporter to test their stochastic dynamics by means of measuring fluorescence intensity of sfGFP using flow cytometry. In the meantime, we inserted the 16nt Csy4 insulator between the promoter and the reporter to stardardize the expression of the reporter downstream by eliminating the influence from 5'UTR. The following chart shows the distribution of sfGFP expression in the circumstance of promoter R0010.

Figure 1. The distribution of the sfGFP expression tested by flow cytometry with promoter R0010 and Csy4 insulator.

Figure 2. The intensity of different promoters is shown by the column height and the randomness of their functions by the bar width.

User Reviews

UNIQ4f10c51b47f4b0a6-partinfo-00000000-QINU UNIQ4f10c51b47f4b0a6-partinfo-00000001-QINU